Next Generation Sequencing (NGS) Workshop

Next Generation Sequencing (NGS) has revolutionized the way we address complex biological questions. The library preparation procedure is one of the major bottlenecks for NGS. This course provides a comprehensive hands-on training on how to prepare high quality libraries for PacBio and Illumina (Solexa) sequencing on the HiSeq2000 or MiSeq platforms. Participants are encouraged to bring their own DNA template and, by the end of the course, will have a library ready for NGS sequencing. Lectures will cover the basic principles of the NGS technology as well as highlight applications such as mRNA-seq, ChIP-seq, de novo sequencing, re-sequencing, mutation discovery, and RNA tagging. NGS technology will be also compared for it's own advantage and disadvantage and the case example of combinatorial usage to meet specific biological goal of sequencing. 

Protocols covered in this course are compatible with multiplexing as well as with the preparation of PCR-free and ribosomal RNA depleted libraries. As sequencing capacities continually increase, automation of library construction is becoming indispensible. We will demonstrate the Apollo324 library automation system. The workshop will conclude with a discussion session focusing on each participant's experimental design to help maximize efficiency and output of sequencing experiments.  Course materials and lunches will be provided.  This course is presented by Dr. Ryan Kim and Dr. Henny O’Geen, DNA Technology Core and Expression Analysis Core at the UC Davis Genome Center.

Registration fee for UC participants                   $450.00

Registration fee for non-UC participants            $650.00

Please email Dr. Kim to register (rwkim@ucdavis.edu).  Enrollment is limited to 12 participants.