Craig J Benham

To make your own construct, you copy & paste the short target DNA sequence (< 5kb) into the provided vector. The insert position starts from 219 (a restriction site). After inserting, click the Submit button to compute the SIDD profile.

Molecular Type: linear dna circular dna

Energetic Type: near neighbor copolymeric

Energy Cutoff:

Name the construct:

Construct*: copy and paste your DNA seqment in between dashed lines:

1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 
61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 
121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg 
181 tcgactctag aggatccccg ggtaccgagc tcgaattc
--------------------------------------------------------------------
	
	( your ins goes here .... )

--------------------------------------------------------------------
gaattccc tatagtgagt cgtattaaat 
241 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
1021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
1081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
1141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
1201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
1261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
1321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
1381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
1441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
1501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
1561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
1621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
1681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
1741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
1801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
1861 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
1921 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
1981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
2041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
2101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
2161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
2221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
2281 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
2341 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
2401 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
2461 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
2521 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga 
2581 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
2641 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
2701 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
2761 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
2821 tttaacgcga attttaacaa aatattaaca aaatattaac gtttacaatt t

*To make your own DNA construct, copy and paste your DNA segment (< 5 kb) in between the dashed lines of the above tex box. Remember: your insert starts from position 219. The rest part is the vector provided.

Set Profile Type:

Output Control:
dna sequence states
parameter set ensemble quantities